Viagra

M

moxie1

Guest
Has anyone looked at the cf foundation website lately? Seems like Viagra might correct some of the CFTR function if CF patients. You never know what might help next. Let's see--Pulmozyme is made from the ovaries of the Chinese hamster. Mucolyxir is from salmon testicles. So of course, why not viagra?


Edited: Sorry, wasn't CF Foundation. This is from the UK.

CYSTIC FIBROSIS

Sildenafil (Viagra) corrects F508-CFTR location in nasal epithelial cells from patients with cystic fibrosis
R L Dormer1 , C M Harris1 , Z Clark1 , M M C Pereira1 , I J M Doull2 , C Norez3 , F Becq3 , M A McPherson1
1 Department of Medical Biochemistry and Immunology, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK
2 Department of Child Health, University Hospital of Wales, Heath Park, Cardiff CF14 4XN, UK
3 Institut de Physiologie et Biologie Cellulaires, UMR 6187, CNRS, Université de Poitiers, France


Correspondence to:
Correspondence to:
Dr R L Dormer
Department of Medical Biochemistry and Immunology, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK; dormer@cardiff.ac.uk

Background: Most patients with cystic fibrosis (CF) have a F508 mutation resulting in abnormal retention of mutant gene protein (F508-CFTR) within the cell. This study was undertaken to investigate F508-CFTR trafficking in native cells from patients with CF with the aim of discovering pharmacological agents that can move F508-CFTR to its correct location in the apical cell membrane.

Method: Nasal epithelial cells were obtained by brushing from individuals with CF. CFTR location was determined using immunofluorescence and confocal imaging in untreated cells and cells treated with sildenafil. The effect of sildenafil treatment on CFTR chloride transport function was measured in CF15 cells using an iodide efflux assay.

Results: In most untreated CF cells F508-CFTR was mislocalised within the cell at a site close to the nucleus. Exposure of cells to sildenafil (2 hours at 37°C) resulted in recruitment of F508-CFTR to the apical membrane and the appearance of chloride transport activity. Sildenafil also increased F508-CFTR trafficking in cells from individuals with CF with a single copy F508 (F508/4016ins) or with a newly described CF trafficking mutation (R1283M).

Conclusions: The findings provide proof of principle for sildenafil as a F508-CFTR trafficking drug and give encouragement for future testing of sildenafil and related PDE5 inhibitors in patients with CF.
 
M

moxie1

Guest
Has anyone looked at the cf foundation website lately? Seems like Viagra might correct some of the CFTR function if CF patients. You never know what might help next. Let's see--Pulmozyme is made from the ovaries of the Chinese hamster. Mucolyxir is from salmon testicles. So of course, why not viagra?


Edited: Sorry, wasn't CF Foundation. This is from the UK.

CYSTIC FIBROSIS

Sildenafil (Viagra) corrects F508-CFTR location in nasal epithelial cells from patients with cystic fibrosis
R L Dormer1 , C M Harris1 , Z Clark1 , M M C Pereira1 , I J M Doull2 , C Norez3 , F Becq3 , M A McPherson1
1 Department of Medical Biochemistry and Immunology, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK
2 Department of Child Health, University Hospital of Wales, Heath Park, Cardiff CF14 4XN, UK
3 Institut de Physiologie et Biologie Cellulaires, UMR 6187, CNRS, Université de Poitiers, France


Correspondence to:
Correspondence to:
Dr R L Dormer
Department of Medical Biochemistry and Immunology, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK; dormer@cardiff.ac.uk

Background: Most patients with cystic fibrosis (CF) have a F508 mutation resulting in abnormal retention of mutant gene protein (F508-CFTR) within the cell. This study was undertaken to investigate F508-CFTR trafficking in native cells from patients with CF with the aim of discovering pharmacological agents that can move F508-CFTR to its correct location in the apical cell membrane.

Method: Nasal epithelial cells were obtained by brushing from individuals with CF. CFTR location was determined using immunofluorescence and confocal imaging in untreated cells and cells treated with sildenafil. The effect of sildenafil treatment on CFTR chloride transport function was measured in CF15 cells using an iodide efflux assay.

Results: In most untreated CF cells F508-CFTR was mislocalised within the cell at a site close to the nucleus. Exposure of cells to sildenafil (2 hours at 37°C) resulted in recruitment of F508-CFTR to the apical membrane and the appearance of chloride transport activity. Sildenafil also increased F508-CFTR trafficking in cells from individuals with CF with a single copy F508 (F508/4016ins) or with a newly described CF trafficking mutation (R1283M).

Conclusions: The findings provide proof of principle for sildenafil as a F508-CFTR trafficking drug and give encouragement for future testing of sildenafil and related PDE5 inhibitors in patients with CF.
 
M

moxie1

Guest
Has anyone looked at the cf foundation website lately? Seems like Viagra might correct some of the CFTR function if CF patients. You never know what might help next. Let's see--Pulmozyme is made from the ovaries of the Chinese hamster. Mucolyxir is from salmon testicles. So of course, why not viagra?


Edited: Sorry, wasn't CF Foundation. This is from the UK.

CYSTIC FIBROSIS

Sildenafil (Viagra) corrects F508-CFTR location in nasal epithelial cells from patients with cystic fibrosis
R L Dormer1 , C M Harris1 , Z Clark1 , M M C Pereira1 , I J M Doull2 , C Norez3 , F Becq3 , M A McPherson1
1 Department of Medical Biochemistry and Immunology, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK
2 Department of Child Health, University Hospital of Wales, Heath Park, Cardiff CF14 4XN, UK
3 Institut de Physiologie et Biologie Cellulaires, UMR 6187, CNRS, Université de Poitiers, France


Correspondence to:
Correspondence to:
Dr R L Dormer
Department of Medical Biochemistry and Immunology, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK; dormer@cardiff.ac.uk

Background: Most patients with cystic fibrosis (CF) have a F508 mutation resulting in abnormal retention of mutant gene protein (F508-CFTR) within the cell. This study was undertaken to investigate F508-CFTR trafficking in native cells from patients with CF with the aim of discovering pharmacological agents that can move F508-CFTR to its correct location in the apical cell membrane.

Method: Nasal epithelial cells were obtained by brushing from individuals with CF. CFTR location was determined using immunofluorescence and confocal imaging in untreated cells and cells treated with sildenafil. The effect of sildenafil treatment on CFTR chloride transport function was measured in CF15 cells using an iodide efflux assay.

Results: In most untreated CF cells F508-CFTR was mislocalised within the cell at a site close to the nucleus. Exposure of cells to sildenafil (2 hours at 37°C) resulted in recruitment of F508-CFTR to the apical membrane and the appearance of chloride transport activity. Sildenafil also increased F508-CFTR trafficking in cells from individuals with CF with a single copy F508 (F508/4016ins) or with a newly described CF trafficking mutation (R1283M).

Conclusions: The findings provide proof of principle for sildenafil as a F508-CFTR trafficking drug and give encouragement for future testing of sildenafil and related PDE5 inhibitors in patients with CF.
 
M

moxie1

Guest
Has anyone looked at the cf foundation website lately? Seems like Viagra might correct some of the CFTR function if CF patients. You never know what might help next. Let's see--Pulmozyme is made from the ovaries of the Chinese hamster. Mucolyxir is from salmon testicles. So of course, why not viagra?


Edited: Sorry, wasn't CF Foundation. This is from the UK.

CYSTIC FIBROSIS

Sildenafil (Viagra) corrects F508-CFTR location in nasal epithelial cells from patients with cystic fibrosis
R L Dormer1 , C M Harris1 , Z Clark1 , M M C Pereira1 , I J M Doull2 , C Norez3 , F Becq3 , M A McPherson1
1 Department of Medical Biochemistry and Immunology, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK
2 Department of Child Health, University Hospital of Wales, Heath Park, Cardiff CF14 4XN, UK
3 Institut de Physiologie et Biologie Cellulaires, UMR 6187, CNRS, Université de Poitiers, France


Correspondence to:
Correspondence to:
Dr R L Dormer
Department of Medical Biochemistry and Immunology, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK; dormer@cardiff.ac.uk

Background: Most patients with cystic fibrosis (CF) have a F508 mutation resulting in abnormal retention of mutant gene protein (F508-CFTR) within the cell. This study was undertaken to investigate F508-CFTR trafficking in native cells from patients with CF with the aim of discovering pharmacological agents that can move F508-CFTR to its correct location in the apical cell membrane.

Method: Nasal epithelial cells were obtained by brushing from individuals with CF. CFTR location was determined using immunofluorescence and confocal imaging in untreated cells and cells treated with sildenafil. The effect of sildenafil treatment on CFTR chloride transport function was measured in CF15 cells using an iodide efflux assay.

Results: In most untreated CF cells F508-CFTR was mislocalised within the cell at a site close to the nucleus. Exposure of cells to sildenafil (2 hours at 37°C) resulted in recruitment of F508-CFTR to the apical membrane and the appearance of chloride transport activity. Sildenafil also increased F508-CFTR trafficking in cells from individuals with CF with a single copy F508 (F508/4016ins) or with a newly described CF trafficking mutation (R1283M).

Conclusions: The findings provide proof of principle for sildenafil as a F508-CFTR trafficking drug and give encouragement for future testing of sildenafil and related PDE5 inhibitors in patients with CF.
 
M

moxie1

Guest
Has anyone looked at the cf foundation website lately? Seems like Viagra might correct some of the CFTR function if CF patients. You never know what might help next. Let's see--Pulmozyme is made from the ovaries of the Chinese hamster. Mucolyxir is from salmon testicles. So of course, why not viagra?


Edited: Sorry, wasn't CF Foundation. This is from the UK.

CYSTIC FIBROSIS

Sildenafil (Viagra) corrects F508-CFTR location in nasal epithelial cells from patients with cystic fibrosis
R L Dormer1 , C M Harris1 , Z Clark1 , M M C Pereira1 , I J M Doull2 , C Norez3 , F Becq3 , M A McPherson1
1 Department of Medical Biochemistry and Immunology, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK
2 Department of Child Health, University Hospital of Wales, Heath Park, Cardiff CF14 4XN, UK
3 Institut de Physiologie et Biologie Cellulaires, UMR 6187, CNRS, Université de Poitiers, France


Correspondence to:
Correspondence to:
Dr R L Dormer
Department of Medical Biochemistry and Immunology, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK; dormer@cardiff.ac.uk

Background: Most patients with cystic fibrosis (CF) have a F508 mutation resulting in abnormal retention of mutant gene protein (F508-CFTR) within the cell. This study was undertaken to investigate F508-CFTR trafficking in native cells from patients with CF with the aim of discovering pharmacological agents that can move F508-CFTR to its correct location in the apical cell membrane.

Method: Nasal epithelial cells were obtained by brushing from individuals with CF. CFTR location was determined using immunofluorescence and confocal imaging in untreated cells and cells treated with sildenafil. The effect of sildenafil treatment on CFTR chloride transport function was measured in CF15 cells using an iodide efflux assay.

Results: In most untreated CF cells F508-CFTR was mislocalised within the cell at a site close to the nucleus. Exposure of cells to sildenafil (2 hours at 37°C) resulted in recruitment of F508-CFTR to the apical membrane and the appearance of chloride transport activity. Sildenafil also increased F508-CFTR trafficking in cells from individuals with CF with a single copy F508 (F508/4016ins) or with a newly described CF trafficking mutation (R1283M).

Conclusions: The findings provide proof of principle for sildenafil as a F508-CFTR trafficking drug and give encouragement for future testing of sildenafil and related PDE5 inhibitors in patients with CF.
 
6

65rosessamurai

Guest
So, the "Guy Thing" was just a side-effect?

Does this mean that females could use it, too?

I'm actually wondering if this is for smaller doses than what the Viagara contains...as I recall from the news, there were a few problems related to the heart.

I wonder what this would do for CF'rs that don't have the F508, or a mutation of one.

Also, if Pulmozyme and Mucolyxir comes from some odd places, I wonder what I'm inhaling with NAC!

Interesting info, though...Thanks for sharing!
 
6

65rosessamurai

Guest
So, the "Guy Thing" was just a side-effect?

Does this mean that females could use it, too?

I'm actually wondering if this is for smaller doses than what the Viagara contains...as I recall from the news, there were a few problems related to the heart.

I wonder what this would do for CF'rs that don't have the F508, or a mutation of one.

Also, if Pulmozyme and Mucolyxir comes from some odd places, I wonder what I'm inhaling with NAC!

Interesting info, though...Thanks for sharing!
 
6

65rosessamurai

Guest
So, the "Guy Thing" was just a side-effect?

Does this mean that females could use it, too?

I'm actually wondering if this is for smaller doses than what the Viagara contains...as I recall from the news, there were a few problems related to the heart.

I wonder what this would do for CF'rs that don't have the F508, or a mutation of one.

Also, if Pulmozyme and Mucolyxir comes from some odd places, I wonder what I'm inhaling with NAC!

Interesting info, though...Thanks for sharing!
 
6

65rosessamurai

Guest
So, the "Guy Thing" was just a side-effect?

Does this mean that females could use it, too?

I'm actually wondering if this is for smaller doses than what the Viagara contains...as I recall from the news, there were a few problems related to the heart.

I wonder what this would do for CF'rs that don't have the F508, or a mutation of one.

Also, if Pulmozyme and Mucolyxir comes from some odd places, I wonder what I'm inhaling with NAC!

Interesting info, though...Thanks for sharing!
 
6

65rosessamurai

Guest
So, the "Guy Thing" was just a side-effect?

Does this mean that females could use it, too?

I'm actually wondering if this is for smaller doses than what the Viagara contains...as I recall from the news, there were a few problems related to the heart.

I wonder what this would do for CF'rs that don't have the F508, or a mutation of one.

Also, if Pulmozyme and Mucolyxir comes from some odd places, I wonder what I'm inhaling with NAC!

Interesting info, though...Thanks for sharing!
 
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