M
MCGrad2006
Guest
Thank you 2perfectboys....im glad im not the only one who cleans the nebs that way.
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eflow
- Thread starter jfarel
- Start date
M
MCGrad2006
Guest
Thank you 2perfectboys....im glad im not the only one who cleans the nebs that way.
M
melleemac
Guest
We boil the boys nebs after each and every treatment for 10 minutes! Why take the chance of using tap water when all you ave to do is boil to sterilize equipment!
Mel
Mel
M
melleemac
Guest
We boil the boys nebs after each and every treatment for 10 minutes! Why take the chance of using tap water when all you ave to do is boil to sterilize equipment!
Mel
Mel
M
melleemac
Guest
We boil the boys nebs after each and every treatment for 10 minutes! Why take the chance of using tap water when all you ave to do is boil to sterilize equipment!
Mel
Mel
JennifersHope
New member
I know a lot of people think PA is everywhere.. The truth is i it is mostly in soil and water. It is common in the hospital setting.. Oddly enough I work in a hosptial and I stilld on't have PA... ODD BALL I KNOW I am....
Bacteria is everywhere though. Staph is way more common than PA.. Staph is all over our skin.. YUCK>>>>
but there has been some studies with the NIH that link PA with tap water.PA is real common in water.. especially distilled water.. PA does not need a whole lot of good conditions for it to fluish and grow.. .. I think that Amy was probably just trying to keep you as healthy as possible, and in an ideal world it would be best to rinse with sterile water.. Actually if you can't afford sterile water.. my friend John used to clean his nebs real good and then pour boiled water over the nebs to clean them... Boiled water for 5 minutes has all the bacteria cleaned...
Pseudomonas aeruginosa strains were tested for the utilization of 47 low-molecular-weight compounds as their sole sources of carbon and energy for growth at a concentration of 2.5 g/liter. Of these compounds, 31 to 35 were consumed. Growth experiments in tap water at 15 degrees C were carried out with one particular strain (P1525) isolated from drinking water. This strain was tested for the utilization of 30 compounds supplied at a concentration of 25 microgram of C per liter. The growth rate (number of generations per hour) of strain P1525 in this tap water was approximately 0.005 h-1, and with 10 compounds it was larger than 0.03 h-1. An average yield of 6.2 x 10(9) colony-forming units per mg of C was obtained from the maximum colony counts (colony-forming units per milliliter). The average yield and maximum colony count of strain P1525 grown in tap water supplied with a mixture of 45 compounds, each at a concentration of 1 microgram of C per liter, enabled us to calculate that 28 compounds were utilized. Growth rates of two P. aeruginosa strains (including P1525) in various types of water at 15 degrees C were half of those of a fluorescent pseudomonad. The concentrations of assimilable organic carbon calculated from maximum colony counts and average yield values amounted to 0.1 to 0.7% of the total organic carbon concentrations in five types of tap water. The assimilable organic carbon percentages were about 10 times larger in river water and in water after ozonation.
Citrate-utilizing bacteria were counted in 289 samples of tap water derived from either surface water or ground water and in 32 samples of raw or partially treated surface water by using media containing ferric ammonium citrate as the carbon and energy source. The citrate-utilizing bacteria constituted only small minorities of the colony counts on Lab-Lemco agar at 25 C in both tap water and surface water. A total of 1071 isolates were obtained, of which 979 were able to utilize citrate. Characterization of the citrate-utilizing isolates revealed that 90% of these bacteria were arginine dihydrolase-positive and belonged to the genera Pseudomonas (84.3%) and Aeromonas (5.7%). The genus Pseudomonas was represented by fluorescent pseudomonads (66.3%), non-fluorescent glucose-utilizing pseudomonads (12.1%) and P. alcaligenes (5.9%). None of the isolates was identified as P. aeruginosa. It is suggested that the pseudomonads and the aeromonads are not adapted to low substrate concentrations. Enumeration of the citrate-utilizing bacteria in tap water therefore may give information on the efficiency of water treatment techniques as regards the substrate removal.
PMID: 596862 [PubMed - indexed for MEDLINE]
Bacteria is everywhere though. Staph is way more common than PA.. Staph is all over our skin.. YUCK>>>>
but there has been some studies with the NIH that link PA with tap water.PA is real common in water.. especially distilled water.. PA does not need a whole lot of good conditions for it to fluish and grow.. .. I think that Amy was probably just trying to keep you as healthy as possible, and in an ideal world it would be best to rinse with sterile water.. Actually if you can't afford sterile water.. my friend John used to clean his nebs real good and then pour boiled water over the nebs to clean them... Boiled water for 5 minutes has all the bacteria cleaned...
Pseudomonas aeruginosa strains were tested for the utilization of 47 low-molecular-weight compounds as their sole sources of carbon and energy for growth at a concentration of 2.5 g/liter. Of these compounds, 31 to 35 were consumed. Growth experiments in tap water at 15 degrees C were carried out with one particular strain (P1525) isolated from drinking water. This strain was tested for the utilization of 30 compounds supplied at a concentration of 25 microgram of C per liter. The growth rate (number of generations per hour) of strain P1525 in this tap water was approximately 0.005 h-1, and with 10 compounds it was larger than 0.03 h-1. An average yield of 6.2 x 10(9) colony-forming units per mg of C was obtained from the maximum colony counts (colony-forming units per milliliter). The average yield and maximum colony count of strain P1525 grown in tap water supplied with a mixture of 45 compounds, each at a concentration of 1 microgram of C per liter, enabled us to calculate that 28 compounds were utilized. Growth rates of two P. aeruginosa strains (including P1525) in various types of water at 15 degrees C were half of those of a fluorescent pseudomonad. The concentrations of assimilable organic carbon calculated from maximum colony counts and average yield values amounted to 0.1 to 0.7% of the total organic carbon concentrations in five types of tap water. The assimilable organic carbon percentages were about 10 times larger in river water and in water after ozonation.
Citrate-utilizing bacteria were counted in 289 samples of tap water derived from either surface water or ground water and in 32 samples of raw or partially treated surface water by using media containing ferric ammonium citrate as the carbon and energy source. The citrate-utilizing bacteria constituted only small minorities of the colony counts on Lab-Lemco agar at 25 C in both tap water and surface water. A total of 1071 isolates were obtained, of which 979 were able to utilize citrate. Characterization of the citrate-utilizing isolates revealed that 90% of these bacteria were arginine dihydrolase-positive and belonged to the genera Pseudomonas (84.3%) and Aeromonas (5.7%). The genus Pseudomonas was represented by fluorescent pseudomonads (66.3%), non-fluorescent glucose-utilizing pseudomonads (12.1%) and P. alcaligenes (5.9%). None of the isolates was identified as P. aeruginosa. It is suggested that the pseudomonads and the aeromonads are not adapted to low substrate concentrations. Enumeration of the citrate-utilizing bacteria in tap water therefore may give information on the efficiency of water treatment techniques as regards the substrate removal.
PMID: 596862 [PubMed - indexed for MEDLINE]
JennifersHope
New member
I know a lot of people think PA is everywhere.. The truth is i it is mostly in soil and water. It is common in the hospital setting.. Oddly enough I work in a hosptial and I stilld on't have PA... ODD BALL I KNOW I am....
Bacteria is everywhere though. Staph is way more common than PA.. Staph is all over our skin.. YUCK>>>>
but there has been some studies with the NIH that link PA with tap water.PA is real common in water.. especially distilled water.. PA does not need a whole lot of good conditions for it to fluish and grow.. .. I think that Amy was probably just trying to keep you as healthy as possible, and in an ideal world it would be best to rinse with sterile water.. Actually if you can't afford sterile water.. my friend John used to clean his nebs real good and then pour boiled water over the nebs to clean them... Boiled water for 5 minutes has all the bacteria cleaned...
Pseudomonas aeruginosa strains were tested for the utilization of 47 low-molecular-weight compounds as their sole sources of carbon and energy for growth at a concentration of 2.5 g/liter. Of these compounds, 31 to 35 were consumed. Growth experiments in tap water at 15 degrees C were carried out with one particular strain (P1525) isolated from drinking water. This strain was tested for the utilization of 30 compounds supplied at a concentration of 25 microgram of C per liter. The growth rate (number of generations per hour) of strain P1525 in this tap water was approximately 0.005 h-1, and with 10 compounds it was larger than 0.03 h-1. An average yield of 6.2 x 10(9) colony-forming units per mg of C was obtained from the maximum colony counts (colony-forming units per milliliter). The average yield and maximum colony count of strain P1525 grown in tap water supplied with a mixture of 45 compounds, each at a concentration of 1 microgram of C per liter, enabled us to calculate that 28 compounds were utilized. Growth rates of two P. aeruginosa strains (including P1525) in various types of water at 15 degrees C were half of those of a fluorescent pseudomonad. The concentrations of assimilable organic carbon calculated from maximum colony counts and average yield values amounted to 0.1 to 0.7% of the total organic carbon concentrations in five types of tap water. The assimilable organic carbon percentages were about 10 times larger in river water and in water after ozonation.
Citrate-utilizing bacteria were counted in 289 samples of tap water derived from either surface water or ground water and in 32 samples of raw or partially treated surface water by using media containing ferric ammonium citrate as the carbon and energy source. The citrate-utilizing bacteria constituted only small minorities of the colony counts on Lab-Lemco agar at 25 C in both tap water and surface water. A total of 1071 isolates were obtained, of which 979 were able to utilize citrate. Characterization of the citrate-utilizing isolates revealed that 90% of these bacteria were arginine dihydrolase-positive and belonged to the genera Pseudomonas (84.3%) and Aeromonas (5.7%). The genus Pseudomonas was represented by fluorescent pseudomonads (66.3%), non-fluorescent glucose-utilizing pseudomonads (12.1%) and P. alcaligenes (5.9%). None of the isolates was identified as P. aeruginosa. It is suggested that the pseudomonads and the aeromonads are not adapted to low substrate concentrations. Enumeration of the citrate-utilizing bacteria in tap water therefore may give information on the efficiency of water treatment techniques as regards the substrate removal.
PMID: 596862 [PubMed - indexed for MEDLINE]
Bacteria is everywhere though. Staph is way more common than PA.. Staph is all over our skin.. YUCK>>>>
but there has been some studies with the NIH that link PA with tap water.PA is real common in water.. especially distilled water.. PA does not need a whole lot of good conditions for it to fluish and grow.. .. I think that Amy was probably just trying to keep you as healthy as possible, and in an ideal world it would be best to rinse with sterile water.. Actually if you can't afford sterile water.. my friend John used to clean his nebs real good and then pour boiled water over the nebs to clean them... Boiled water for 5 minutes has all the bacteria cleaned...
Pseudomonas aeruginosa strains were tested for the utilization of 47 low-molecular-weight compounds as their sole sources of carbon and energy for growth at a concentration of 2.5 g/liter. Of these compounds, 31 to 35 were consumed. Growth experiments in tap water at 15 degrees C were carried out with one particular strain (P1525) isolated from drinking water. This strain was tested for the utilization of 30 compounds supplied at a concentration of 25 microgram of C per liter. The growth rate (number of generations per hour) of strain P1525 in this tap water was approximately 0.005 h-1, and with 10 compounds it was larger than 0.03 h-1. An average yield of 6.2 x 10(9) colony-forming units per mg of C was obtained from the maximum colony counts (colony-forming units per milliliter). The average yield and maximum colony count of strain P1525 grown in tap water supplied with a mixture of 45 compounds, each at a concentration of 1 microgram of C per liter, enabled us to calculate that 28 compounds were utilized. Growth rates of two P. aeruginosa strains (including P1525) in various types of water at 15 degrees C were half of those of a fluorescent pseudomonad. The concentrations of assimilable organic carbon calculated from maximum colony counts and average yield values amounted to 0.1 to 0.7% of the total organic carbon concentrations in five types of tap water. The assimilable organic carbon percentages were about 10 times larger in river water and in water after ozonation.
Citrate-utilizing bacteria were counted in 289 samples of tap water derived from either surface water or ground water and in 32 samples of raw or partially treated surface water by using media containing ferric ammonium citrate as the carbon and energy source. The citrate-utilizing bacteria constituted only small minorities of the colony counts on Lab-Lemco agar at 25 C in both tap water and surface water. A total of 1071 isolates were obtained, of which 979 were able to utilize citrate. Characterization of the citrate-utilizing isolates revealed that 90% of these bacteria were arginine dihydrolase-positive and belonged to the genera Pseudomonas (84.3%) and Aeromonas (5.7%). The genus Pseudomonas was represented by fluorescent pseudomonads (66.3%), non-fluorescent glucose-utilizing pseudomonads (12.1%) and P. alcaligenes (5.9%). None of the isolates was identified as P. aeruginosa. It is suggested that the pseudomonads and the aeromonads are not adapted to low substrate concentrations. Enumeration of the citrate-utilizing bacteria in tap water therefore may give information on the efficiency of water treatment techniques as regards the substrate removal.
PMID: 596862 [PubMed - indexed for MEDLINE]
JennifersHope
New member
I know a lot of people think PA is everywhere.. The truth is i it is mostly in soil and water. It is common in the hospital setting.. Oddly enough I work in a hosptial and I stilld on't have PA... ODD BALL I KNOW I am....
Bacteria is everywhere though. Staph is way more common than PA.. Staph is all over our skin.. YUCK>>>>
but there has been some studies with the NIH that link PA with tap water.PA is real common in water.. especially distilled water.. PA does not need a whole lot of good conditions for it to fluish and grow.. .. I think that Amy was probably just trying to keep you as healthy as possible, and in an ideal world it would be best to rinse with sterile water.. Actually if you can't afford sterile water.. my friend John used to clean his nebs real good and then pour boiled water over the nebs to clean them... Boiled water for 5 minutes has all the bacteria cleaned...
Pseudomonas aeruginosa strains were tested for the utilization of 47 low-molecular-weight compounds as their sole sources of carbon and energy for growth at a concentration of 2.5 g/liter. Of these compounds, 31 to 35 were consumed. Growth experiments in tap water at 15 degrees C were carried out with one particular strain (P1525) isolated from drinking water. This strain was tested for the utilization of 30 compounds supplied at a concentration of 25 microgram of C per liter. The growth rate (number of generations per hour) of strain P1525 in this tap water was approximately 0.005 h-1, and with 10 compounds it was larger than 0.03 h-1. An average yield of 6.2 x 10(9) colony-forming units per mg of C was obtained from the maximum colony counts (colony-forming units per milliliter). The average yield and maximum colony count of strain P1525 grown in tap water supplied with a mixture of 45 compounds, each at a concentration of 1 microgram of C per liter, enabled us to calculate that 28 compounds were utilized. Growth rates of two P. aeruginosa strains (including P1525) in various types of water at 15 degrees C were half of those of a fluorescent pseudomonad. The concentrations of assimilable organic carbon calculated from maximum colony counts and average yield values amounted to 0.1 to 0.7% of the total organic carbon concentrations in five types of tap water. The assimilable organic carbon percentages were about 10 times larger in river water and in water after ozonation.
Citrate-utilizing bacteria were counted in 289 samples of tap water derived from either surface water or ground water and in 32 samples of raw or partially treated surface water by using media containing ferric ammonium citrate as the carbon and energy source. The citrate-utilizing bacteria constituted only small minorities of the colony counts on Lab-Lemco agar at 25 C in both tap water and surface water. A total of 1071 isolates were obtained, of which 979 were able to utilize citrate. Characterization of the citrate-utilizing isolates revealed that 90% of these bacteria were arginine dihydrolase-positive and belonged to the genera Pseudomonas (84.3%) and Aeromonas (5.7%). The genus Pseudomonas was represented by fluorescent pseudomonads (66.3%), non-fluorescent glucose-utilizing pseudomonads (12.1%) and P. alcaligenes (5.9%). None of the isolates was identified as P. aeruginosa. It is suggested that the pseudomonads and the aeromonads are not adapted to low substrate concentrations. Enumeration of the citrate-utilizing bacteria in tap water therefore may give information on the efficiency of water treatment techniques as regards the substrate removal.
PMID: 596862 [PubMed - indexed for MEDLINE]
Bacteria is everywhere though. Staph is way more common than PA.. Staph is all over our skin.. YUCK>>>>
but there has been some studies with the NIH that link PA with tap water.PA is real common in water.. especially distilled water.. PA does not need a whole lot of good conditions for it to fluish and grow.. .. I think that Amy was probably just trying to keep you as healthy as possible, and in an ideal world it would be best to rinse with sterile water.. Actually if you can't afford sterile water.. my friend John used to clean his nebs real good and then pour boiled water over the nebs to clean them... Boiled water for 5 minutes has all the bacteria cleaned...
Pseudomonas aeruginosa strains were tested for the utilization of 47 low-molecular-weight compounds as their sole sources of carbon and energy for growth at a concentration of 2.5 g/liter. Of these compounds, 31 to 35 were consumed. Growth experiments in tap water at 15 degrees C were carried out with one particular strain (P1525) isolated from drinking water. This strain was tested for the utilization of 30 compounds supplied at a concentration of 25 microgram of C per liter. The growth rate (number of generations per hour) of strain P1525 in this tap water was approximately 0.005 h-1, and with 10 compounds it was larger than 0.03 h-1. An average yield of 6.2 x 10(9) colony-forming units per mg of C was obtained from the maximum colony counts (colony-forming units per milliliter). The average yield and maximum colony count of strain P1525 grown in tap water supplied with a mixture of 45 compounds, each at a concentration of 1 microgram of C per liter, enabled us to calculate that 28 compounds were utilized. Growth rates of two P. aeruginosa strains (including P1525) in various types of water at 15 degrees C were half of those of a fluorescent pseudomonad. The concentrations of assimilable organic carbon calculated from maximum colony counts and average yield values amounted to 0.1 to 0.7% of the total organic carbon concentrations in five types of tap water. The assimilable organic carbon percentages were about 10 times larger in river water and in water after ozonation.
Citrate-utilizing bacteria were counted in 289 samples of tap water derived from either surface water or ground water and in 32 samples of raw or partially treated surface water by using media containing ferric ammonium citrate as the carbon and energy source. The citrate-utilizing bacteria constituted only small minorities of the colony counts on Lab-Lemco agar at 25 C in both tap water and surface water. A total of 1071 isolates were obtained, of which 979 were able to utilize citrate. Characterization of the citrate-utilizing isolates revealed that 90% of these bacteria were arginine dihydrolase-positive and belonged to the genera Pseudomonas (84.3%) and Aeromonas (5.7%). The genus Pseudomonas was represented by fluorescent pseudomonads (66.3%), non-fluorescent glucose-utilizing pseudomonads (12.1%) and P. alcaligenes (5.9%). None of the isolates was identified as P. aeruginosa. It is suggested that the pseudomonads and the aeromonads are not adapted to low substrate concentrations. Enumeration of the citrate-utilizing bacteria in tap water therefore may give information on the efficiency of water treatment techniques as regards the substrate removal.
PMID: 596862 [PubMed - indexed for MEDLINE]
here in the uk, lots of us who use eflows have baby sterilisers and after each treatment just take it apart and put it in there! job done! the steam is sterile and distilled as its been boiled. pari in the uk have given the green light to use this method and my cf team reccomennded it to me when i first got it. it must be an electrical one and not a microwave one. this might help you.
here in the uk, lots of us who use eflows have baby sterilisers and after each treatment just take it apart and put it in there! job done! the steam is sterile and distilled as its been boiled. pari in the uk have given the green light to use this method and my cf team reccomennded it to me when i first got it. it must be an electrical one and not a microwave one. this might help you.
here in the uk, lots of us who use eflows have baby sterilisers and after each treatment just take it apart and put it in there! job done! the steam is sterile and distilled as its been boiled. pari in the uk have given the green light to use this method and my cf team reccomennded it to me when i first got it. it must be an electrical one and not a microwave one. this might help you.
2
2perfectboys
Guest
I don't think the eflow is anymore time consuming to clean then other nebulizers. They all need to be cleaned after using. Soap with tap water is sufficent as it is cleaning off the tiny holes in the equipment. You are suppose to soak the mouthpiece in the cleaning soluntion also. How did PA get deep into your lungs, if not through mouth which major opening did it invade?
PA can also be found in the air, hence prob how a lot of people got infected with it at the old CF camps. So I guess Amy u should not breath on your eflow when u get it, cause u will just be putting more PA into your deep lungs.
PA can also be found in the air, hence prob how a lot of people got infected with it at the old CF camps. So I guess Amy u should not breath on your eflow when u get it, cause u will just be putting more PA into your deep lungs.
2
2perfectboys
Guest
I don't think the eflow is anymore time consuming to clean then other nebulizers. They all need to be cleaned after using. Soap with tap water is sufficent as it is cleaning off the tiny holes in the equipment. You are suppose to soak the mouthpiece in the cleaning soluntion also. How did PA get deep into your lungs, if not through mouth which major opening did it invade?
PA can also be found in the air, hence prob how a lot of people got infected with it at the old CF camps. So I guess Amy u should not breath on your eflow when u get it, cause u will just be putting more PA into your deep lungs.
PA can also be found in the air, hence prob how a lot of people got infected with it at the old CF camps. So I guess Amy u should not breath on your eflow when u get it, cause u will just be putting more PA into your deep lungs.
2
2perfectboys
Guest
I don't think the eflow is anymore time consuming to clean then other nebulizers. They all need to be cleaned after using. Soap with tap water is sufficent as it is cleaning off the tiny holes in the equipment. You are suppose to soak the mouthpiece in the cleaning soluntion also. How did PA get deep into your lungs, if not through mouth which major opening did it invade?
PA can also be found in the air, hence prob how a lot of people got infected with it at the old CF camps. So I guess Amy u should not breath on your eflow when u get it, cause u will just be putting more PA into your deep lungs.
PA can also be found in the air, hence prob how a lot of people got infected with it at the old CF camps. So I guess Amy u should not breath on your eflow when u get it, cause u will just be putting more PA into your deep lungs.