How to read a sputum culture

Asexyblond23

New member
Well I got a copy of my latest suptum culture and I am confused on what exaclty everything means. For example it says 2+ gram negative rods, 2+ gram positive cocci. Is the 2+ good or bad? Last culture they were both 3+. Q score = 0, last culture it was q score = -2. Lat time it was no poyls seen and this time it was 1+ polys, what the heck are polys? I also went from light to moderete growth of pseudo so thats nothing to whoo hoo over <img src="i/expressions/face-icon-small-smile.gif" border="0"> But at least this time the growth of upper respiratpry bacteria went from heavy to moderete growth. So is there anyone that can help me out on reading these numbers and helping me understand what is good and what is bad?
 

Asexyblond23

New member
Well I got a copy of my latest suptum culture and I am confused on what exaclty everything means. For example it says 2+ gram negative rods, 2+ gram positive cocci. Is the 2+ good or bad? Last culture they were both 3+. Q score = 0, last culture it was q score = -2. Lat time it was no poyls seen and this time it was 1+ polys, what the heck are polys? I also went from light to moderete growth of pseudo so thats nothing to whoo hoo over <img src="i/expressions/face-icon-small-smile.gif" border="0"> But at least this time the growth of upper respiratpry bacteria went from heavy to moderete growth. So is there anyone that can help me out on reading these numbers and helping me understand what is good and what is bad?
 

Asexyblond23

New member
Well I got a copy of my latest suptum culture and I am confused on what exaclty everything means. For example it says 2+ gram negative rods, 2+ gram positive cocci. Is the 2+ good or bad? Last culture they were both 3+. Q score = 0, last culture it was q score = -2. Lat time it was no poyls seen and this time it was 1+ polys, what the heck are polys? I also went from light to moderete growth of pseudo so thats nothing to whoo hoo over <img src="i/expressions/face-icon-small-smile.gif" border="0"> But at least this time the growth of upper respiratpry bacteria went from heavy to moderete growth. So is there anyone that can help me out on reading these numbers and helping me understand what is good and what is bad?
 

Asexyblond23

New member
Well I got a copy of my latest suptum culture and I am confused on what exaclty everything means. For example it says 2+ gram negative rods, 2+ gram positive cocci. Is the 2+ good or bad? Last culture they were both 3+. Q score = 0, last culture it was q score = -2. Lat time it was no poyls seen and this time it was 1+ polys, what the heck are polys? I also went from light to moderete growth of pseudo so thats nothing to whoo hoo over <img src="i/expressions/face-icon-small-smile.gif" border="0"> But at least this time the growth of upper respiratpry bacteria went from heavy to moderete growth. So is there anyone that can help me out on reading these numbers and helping me understand what is good and what is bad?
 

Asexyblond23

New member
Well I got a copy of my latest suptum culture and I am confused on what exaclty everything means. For example it says 2+ gram negative rods, 2+ gram positive cocci. Is the 2+ good or bad? Last culture they were both 3+. Q score = 0, last culture it was q score = -2. Lat time it was no poyls seen and this time it was 1+ polys, what the heck are polys? I also went from light to moderete growth of pseudo so thats nothing to whoo hoo over <img src="i/expressions/face-icon-small-smile.gif" border="0"> But at least this time the growth of upper respiratpry bacteria went from heavy to moderete growth. So is there anyone that can help me out on reading these numbers and helping me understand what is good and what is bad?
 

Asexyblond23

New member
Well I got a copy of my latest suptum culture and I am confused on what exaclty everything means. For example it says 2+ gram negative rods, 2+ gram positive cocci. Is the 2+ good or bad? Last culture they were both 3+. Q score = 0, last culture it was q score = -2. Lat time it was no poyls seen and this time it was 1+ polys, what the heck are polys? I also went from light to moderete growth of pseudo so thats nothing to whoo hoo over <img src="i/expressions/face-icon-small-smile.gif" border="0"> But at least this time the growth of upper respiratpry bacteria went from heavy to moderete growth. So is there anyone that can help me out on reading these numbers and helping me understand what is good and what is bad?
 

lightNlife

New member
Hi Alisha,

All this is stuff that pretty much just refers to the lab procedure involved in analyzing a sputum culture. Basically you're not any better off knowing what these numbers mean, but since you asked, I'll do my best to explain.

<b>Q score</b> is a ratio of squamous cells to neutrophils. Squamous cells are flat cells (they look like fish scales under the microscope; neutrophils are a type of white blood cells that the body deploys to fight bacteria.)

<b>The numbers 2+ </b>etc. Indicate progressively higher numbers of cells visible. Sometimes it takes a different type of stain to detect the cells. See cepaciagal's explanation below.

<b>Polys</b> are neutrophils. The long name is polymorphonuclear leukocytes. They appear blue when Gram stained.

<b>
Gram positive</b> means the bacteria turn blue when stained by a particular chemical.

<b>Gram negative</b> means the bacteria turn red when stained by a particular chemical.
 

lightNlife

New member
Hi Alisha,

All this is stuff that pretty much just refers to the lab procedure involved in analyzing a sputum culture. Basically you're not any better off knowing what these numbers mean, but since you asked, I'll do my best to explain.

<b>Q score</b> is a ratio of squamous cells to neutrophils. Squamous cells are flat cells (they look like fish scales under the microscope; neutrophils are a type of white blood cells that the body deploys to fight bacteria.)

<b>The numbers 2+ </b>etc. Indicate progressively higher numbers of cells visible. Sometimes it takes a different type of stain to detect the cells. See cepaciagal's explanation below.

<b>Polys</b> are neutrophils. The long name is polymorphonuclear leukocytes. They appear blue when Gram stained.

<b>
Gram positive</b> means the bacteria turn blue when stained by a particular chemical.

<b>Gram negative</b> means the bacteria turn red when stained by a particular chemical.
 

lightNlife

New member
Hi Alisha,

All this is stuff that pretty much just refers to the lab procedure involved in analyzing a sputum culture. Basically you're not any better off knowing what these numbers mean, but since you asked, I'll do my best to explain.

<b>Q score</b> is a ratio of squamous cells to neutrophils. Squamous cells are flat cells (they look like fish scales under the microscope; neutrophils are a type of white blood cells that the body deploys to fight bacteria.)

<b>The numbers 2+ </b>etc. Indicate progressively higher numbers of cells visible. Sometimes it takes a different type of stain to detect the cells. See cepaciagal's explanation below.

<b>Polys</b> are neutrophils. The long name is polymorphonuclear leukocytes. They appear blue when Gram stained.

<b>
Gram positive</b> means the bacteria turn blue when stained by a particular chemical.

<b>Gram negative</b> means the bacteria turn red when stained by a particular chemical.
 

lightNlife

New member
Hi Alisha,

All this is stuff that pretty much just refers to the lab procedure involved in analyzing a sputum culture. Basically you're not any better off knowing what these numbers mean, but since you asked, I'll do my best to explain.

<b>Q score</b> is a ratio of squamous cells to neutrophils. Squamous cells are flat cells (they look like fish scales under the microscope; neutrophils are a type of white blood cells that the body deploys to fight bacteria.)

<b>The numbers 2+ </b>etc. Indicate progressively higher numbers of cells visible. Sometimes it takes a different type of stain to detect the cells. See cepaciagal's explanation below.

<b>Polys</b> are neutrophils. The long name is polymorphonuclear leukocytes. They appear blue when Gram stained.

<b>
Gram positive</b> means the bacteria turn blue when stained by a particular chemical.

<b>Gram negative</b> means the bacteria turn red when stained by a particular chemical.
 

lightNlife

New member
Hi Alisha,

All this is stuff that pretty much just refers to the lab procedure involved in analyzing a sputum culture. Basically you're not any better off knowing what these numbers mean, but since you asked, I'll do my best to explain.

<b>Q score</b> is a ratio of squamous cells to neutrophils. Squamous cells are flat cells (they look like fish scales under the microscope; neutrophils are a type of white blood cells that the body deploys to fight bacteria.)

<b>The numbers 2+ </b>etc. Indicate progressively higher numbers of cells visible. Sometimes it takes a different type of stain to detect the cells. See cepaciagal's explanation below.

<b>Polys</b> are neutrophils. The long name is polymorphonuclear leukocytes. They appear blue when Gram stained.

<b>
Gram positive</b> means the bacteria turn blue when stained by a particular chemical.

<b>Gram negative</b> means the bacteria turn red when stained by a particular chemical.
 

lightNlife

New member
Hi Alisha,

All this is stuff that pretty much just refers to the lab procedure involved in analyzing a sputum culture. Basically you're not any better off knowing what these numbers mean, but since you asked, I'll do my best to explain.

<b>Q score</b> is a ratio of squamous cells to neutrophils. Squamous cells are flat cells (they look like fish scales under the microscope; neutrophils are a type of white blood cells that the body deploys to fight bacteria.)

<b>The numbers 2+ </b>etc. Indicate progressively higher numbers of cells visible. Sometimes it takes a different type of stain to detect the cells. See cepaciagal's explanation below.

<b>Polys</b> are neutrophils. The long name is polymorphonuclear leukocytes. They appear blue when Gram stained.

<b>
Gram positive</b> means the bacteria turn blue when stained by a particular chemical.

<b>Gram negative</b> means the bacteria turn red when stained by a particular chemical.
 

Asexyblond23

New member
Wow that was an amazing explanation!!! Thank you so much! So is it better to have a higer number or a lower number? Or does it not matter?
 

Asexyblond23

New member
Wow that was an amazing explanation!!! Thank you so much! So is it better to have a higer number or a lower number? Or does it not matter?
 

Asexyblond23

New member
Wow that was an amazing explanation!!! Thank you so much! So is it better to have a higer number or a lower number? Or does it not matter?
 

Asexyblond23

New member
Wow that was an amazing explanation!!! Thank you so much! So is it better to have a higer number or a lower number? Or does it not matter?
 

Asexyblond23

New member
Wow that was an amazing explanation!!! Thank you so much! So is it better to have a higer number or a lower number? Or does it not matter?
 

Asexyblond23

New member
Wow that was an amazing explanation!!! Thank you so much! So is it better to have a higer number or a lower number? Or does it not matter?
 

JustDucky

New member
Hi there! As far as numbers go...when you see 1+, 2+ etc up to 4+ it is telling you how much bacteria is there, 4+ being the highest. The a lower value (ie 1+) correlates with a lesser amount of bacteria visualized than 4+ which would be significantly greater You also mentioned polys...they are also called white blood cells, sometimes on a culture you will see WBC instead of polys written. Epithelial cells are cells that slough off your respiratory tract. WBC's are significant for infection. They too use the number system from 0 to 4+, it also depends on how many cells the micros see under a microscope in a set field.
0=none seen
1+= 1 to 5 cells seen
2+= 5 to 10 cells
3+= 11 to 25
4+= >25 cells

Here's an example of an old result of mine.
Analysis for Cystic Fibrosis sputum (which means they use different types of plates and conditions to grow organisms specific to CF that could be missed with the usual culture techniques)

Examination (under the microscope before culturing)
3+ WBC
<10 squamous epithelial cells
2+ gram negative bacilli (bacilli, cocci refer to the shape, bacilli are rod shaped, cocci are round)
2+ gram positive cocci

Final results (culture):
3+ Burkholderia cepacia (3+ means that there was growth into the second quad of a petri dish with >30 colonies..)
2+ Staphyloccus aureus (2+ means the micro saw 11-30 colonies on the petri dish)
1+ yeast (1+ micro saw 1-10)

On my samples there is always sensitivities which tell you what the bacteria are sensitive to antibiotic wise. It helps the docs choose the correct antibiotics to treat me during an active infection.

Other things you might see on your cultures:
Normal flora- means organisms that are typically found in the environment tested
hyphae- seen in fungal
acid fast bacilli- result seen with the Mycobacterium species (examples are M. avium complex and TB)

I am sure there are others, but I can't think of them at this moment.

I hope I've helped, Hugs Jenn <img src="i/expressions/face-icon-small-smile.gif" border="0">
 

JustDucky

New member
Hi there! As far as numbers go...when you see 1+, 2+ etc up to 4+ it is telling you how much bacteria is there, 4+ being the highest. The a lower value (ie 1+) correlates with a lesser amount of bacteria visualized than 4+ which would be significantly greater You also mentioned polys...they are also called white blood cells, sometimes on a culture you will see WBC instead of polys written. Epithelial cells are cells that slough off your respiratory tract. WBC's are significant for infection. They too use the number system from 0 to 4+, it also depends on how many cells the micros see under a microscope in a set field.
0=none seen
1+= 1 to 5 cells seen
2+= 5 to 10 cells
3+= 11 to 25
4+= >25 cells

Here's an example of an old result of mine.
Analysis for Cystic Fibrosis sputum (which means they use different types of plates and conditions to grow organisms specific to CF that could be missed with the usual culture techniques)

Examination (under the microscope before culturing)
3+ WBC
<10 squamous epithelial cells
2+ gram negative bacilli (bacilli, cocci refer to the shape, bacilli are rod shaped, cocci are round)
2+ gram positive cocci

Final results (culture):
3+ Burkholderia cepacia (3+ means that there was growth into the second quad of a petri dish with >30 colonies..)
2+ Staphyloccus aureus (2+ means the micro saw 11-30 colonies on the petri dish)
1+ yeast (1+ micro saw 1-10)

On my samples there is always sensitivities which tell you what the bacteria are sensitive to antibiotic wise. It helps the docs choose the correct antibiotics to treat me during an active infection.

Other things you might see on your cultures:
Normal flora- means organisms that are typically found in the environment tested
hyphae- seen in fungal
acid fast bacilli- result seen with the Mycobacterium species (examples are M. avium complex and TB)

I am sure there are others, but I can't think of them at this moment.

I hope I've helped, Hugs Jenn <img src="i/expressions/face-icon-small-smile.gif" border="0">
 
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