I have recently been studying L-Arginine and its role in CF. L-arginine is a substrate for Nitric Oxide and is believed to be deficient in CF patients.
<i>Nitric Oxide is important for its role in cell signaling,
facilitating the building of muscle tissue, and the uptake of
nutrients and glucose by muscle tissue where it is used for energy
(1, 8). Additionally, NO is responsible for smooth muscle
relaxation and regulating ciliary beat function (1). <b>NO is also
responsible for increased conductance of the CFTR protein, enhancing
chloride transport while down-regulating sodium absorption </b>(1, 3, 6,
7) resulting in increased hydration of endobronchial secretions,
facilitating elimination of bacteria from the lungs (1,7,19). NO
may also decrease neutrophil accumulation in the lung (20, 21).
Finally, NO is known to act as a bactericidal agent against
two of the most prevalent pathogens common in the pathology of
Cystic Fibrosis, Pseudomonas Aeruginosa (PA) and Staphylococcus
Aerus (SA) (1, 18). The bactericidal effects of NO work first by
reducing adherence of bacteria in the CF lung, and second by
disrupting microbial functions by interacting with critical
membranes and cellular thiols in bacteria (23, 24, 25). As
adherence is the initial step in establishing infection, augmenting
NO very early in CF would in theory have therapeutic effects (3).</i>
<b>http://members.tripod.com/uvicf/research/ArginineGSNO.htm</b>
I encourage you to read that whole article as it is VERY interesting and has some very important implications if proven correct.
As a result of the research I have done, I have started inhaling the L-Arginine immediately after I inhale GSH. This will form GSNO in the lungs (refer to article).
GSNO acts as a reserve for both GSH and NO (Nitric Oxide) which are both deficient in the lungs of CF patients.
"Low levels of GSNO in CF airways would seem to account for the affinity of the CF lung to colonize SA and PA since a lack of NO is recognized for increased adherence of bacteria in the CF lung (1, 3, 5, 7). It is reasonable to suggest, therefore, that normalizing airway GSNO in CF will increase host defense against these common CF pathogens."
This next part is taken from: <a target=_blank class=ftalternatingbarlinklarge href="http://health.groups.yahoo.com/group/wlgroup2/message/100">http://health.groups.yahoo.com...p/wlgroup2/message/100</a> , and is an account from someone who has already inhaled L-arginine in the same way that they did in the study.
<i>Inhaling Buffered L-arginine
1) Be careful to be sure to mix it in the proper proportions, as
inhaling it at the wrong pH could pose a problem.
2) To make the solution, I dissolved 0.9g of l-arginine free-base and
15g of l-arginine hydrochloride in 300ml of purified water. (Solomons
used tap water.) This mixture should have a pH of 7.4-7.6 and should be
refrigerated. In Solomon's study, it was inhaled four times a day for thirty
<b>3) 16-24 hours after I began inhaling it I became extremely congested,
more congested than when I'm sick, and was coughing up the most vile
looking, foulest smelling mucus. Even when I've been sick, it doesn't look
that gross! And "coughing up" is an overstatement: mostly, it just slid out
of it's own accord. This continued for a couple of days. My breath smelled
REALLY bad, too, but this also went away.</b>
4) After inhaling it for a long time, I am sometimes short of breath
for 15-30 minutes afterward, but have found that adding 0.5cc of Ventolin
alleviates this(though I rarely do it).
<b>5) Oral use resulted in weight gain, better fat absorption, and 100%
success in relieving ALL intestinal cramping.</b>
6) The study also noted that, to achieve it's preventative effects, it
might be sufficient to take l-arginine orally, as a small amount ends up in
the bronchial fluids.
7) Solomons wrote a rebuttal in Pediatrics, 1976, vol. 76, p. 166 where
he criticized the methodology of the East German study, arguing that the
researchers should have dissolved the l-arginine in water, instead of sodium
hydroxide, and that this could actually cause lung inflammation and that
arginine needed to buffered by its own salt.
7) l-arginine should probably not be mixed with saline</i>
I've researched many articles on the subject but don't have the time at the moment to cite them.
I am not entirely sure about what GSH does in the body, but if someone were to supplement GSH and not L-Arginine, then a much smaller benefit is likely. Due to the amount of very promising success stories surrounding GSH (<b>http://members5.boardhost.com/CFGSH/</b>) Adding in L-arginine both internally and inhaled to GSH will increase GSNO in the body/lungs and have promising results.
Does anyone have any experience with this?
I started this last night and have since done a dose this morning, I will continue once in the morning and once in the night for a couple weeks and let you guys know how it works out.
I have ordered the l-arginine that you take internally and will start it as soon as it arrives (25grams/day)
Jono
ps. read the article
pss I appologise if this wasn't very cohesively written, I just jotted it all down as it was fresh in my memory.
<i>Nitric Oxide is important for its role in cell signaling,
facilitating the building of muscle tissue, and the uptake of
nutrients and glucose by muscle tissue where it is used for energy
(1, 8). Additionally, NO is responsible for smooth muscle
relaxation and regulating ciliary beat function (1). <b>NO is also
responsible for increased conductance of the CFTR protein, enhancing
chloride transport while down-regulating sodium absorption </b>(1, 3, 6,
7) resulting in increased hydration of endobronchial secretions,
facilitating elimination of bacteria from the lungs (1,7,19). NO
may also decrease neutrophil accumulation in the lung (20, 21).
Finally, NO is known to act as a bactericidal agent against
two of the most prevalent pathogens common in the pathology of
Cystic Fibrosis, Pseudomonas Aeruginosa (PA) and Staphylococcus
Aerus (SA) (1, 18). The bactericidal effects of NO work first by
reducing adherence of bacteria in the CF lung, and second by
disrupting microbial functions by interacting with critical
membranes and cellular thiols in bacteria (23, 24, 25). As
adherence is the initial step in establishing infection, augmenting
NO very early in CF would in theory have therapeutic effects (3).</i>
<b>http://members.tripod.com/uvicf/research/ArginineGSNO.htm</b>
I encourage you to read that whole article as it is VERY interesting and has some very important implications if proven correct.
As a result of the research I have done, I have started inhaling the L-Arginine immediately after I inhale GSH. This will form GSNO in the lungs (refer to article).
GSNO acts as a reserve for both GSH and NO (Nitric Oxide) which are both deficient in the lungs of CF patients.
"Low levels of GSNO in CF airways would seem to account for the affinity of the CF lung to colonize SA and PA since a lack of NO is recognized for increased adherence of bacteria in the CF lung (1, 3, 5, 7). It is reasonable to suggest, therefore, that normalizing airway GSNO in CF will increase host defense against these common CF pathogens."
This next part is taken from: <a target=_blank class=ftalternatingbarlinklarge href="http://health.groups.yahoo.com/group/wlgroup2/message/100">http://health.groups.yahoo.com...p/wlgroup2/message/100</a> , and is an account from someone who has already inhaled L-arginine in the same way that they did in the study.
<i>Inhaling Buffered L-arginine
1) Be careful to be sure to mix it in the proper proportions, as
inhaling it at the wrong pH could pose a problem.
2) To make the solution, I dissolved 0.9g of l-arginine free-base and
15g of l-arginine hydrochloride in 300ml of purified water. (Solomons
used tap water.) This mixture should have a pH of 7.4-7.6 and should be
refrigerated. In Solomon's study, it was inhaled four times a day for thirty
<b>3) 16-24 hours after I began inhaling it I became extremely congested,
more congested than when I'm sick, and was coughing up the most vile
looking, foulest smelling mucus. Even when I've been sick, it doesn't look
that gross! And "coughing up" is an overstatement: mostly, it just slid out
of it's own accord. This continued for a couple of days. My breath smelled
REALLY bad, too, but this also went away.</b>
4) After inhaling it for a long time, I am sometimes short of breath
for 15-30 minutes afterward, but have found that adding 0.5cc of Ventolin
alleviates this(though I rarely do it).
<b>5) Oral use resulted in weight gain, better fat absorption, and 100%
success in relieving ALL intestinal cramping.</b>
6) The study also noted that, to achieve it's preventative effects, it
might be sufficient to take l-arginine orally, as a small amount ends up in
the bronchial fluids.
7) Solomons wrote a rebuttal in Pediatrics, 1976, vol. 76, p. 166 where
he criticized the methodology of the East German study, arguing that the
researchers should have dissolved the l-arginine in water, instead of sodium
hydroxide, and that this could actually cause lung inflammation and that
arginine needed to buffered by its own salt.
7) l-arginine should probably not be mixed with saline</i>
I've researched many articles on the subject but don't have the time at the moment to cite them.
I am not entirely sure about what GSH does in the body, but if someone were to supplement GSH and not L-Arginine, then a much smaller benefit is likely. Due to the amount of very promising success stories surrounding GSH (<b>http://members5.boardhost.com/CFGSH/</b>) Adding in L-arginine both internally and inhaled to GSH will increase GSNO in the body/lungs and have promising results.
Does anyone have any experience with this?
I started this last night and have since done a dose this morning, I will continue once in the morning and once in the night for a couple weeks and let you guys know how it works out.
I have ordered the l-arginine that you take internally and will start it as soon as it arrives (25grams/day)
Jono
ps. read the article
pss I appologise if this wasn't very cohesively written, I just jotted it all down as it was fresh in my memory.