My son cultured positive for PA in Dec 05 (they said it was a very small amount) and then Cultured neg for PA in Jan 06 (before we started TOBI). Our doctor still put him on the 6 month (28 days on, 28 off) round of TOBI. My son was 4 at the time and he said that it is not always easy to get a good culture on the little ones. He said that could be the reason for the positive then negative culture. He said there are many other reasons, one of them being that my son could have just eaten something with PA on it (it is everywhere) such as fruit and the culture picked that up.